Lumify muse bar3/18/2023 ![]() We used low BAK levels, because we found that 0.5 mg/ml and 50 μg/ml BAK killed iHMGECs within 1 day after a 15 min exposure. iHMGECs, iHCECs and iHConjECs were cultured with different concentrations of BAK (5 μg/ml to 0.005 μg/ml) or FA (1 mg/ml to 1 μg/ml) under basal, proliferating or differentiating conditions up to 7 days. Accordingly, we tested the influence of BAK and FA on the morphology, survival, and proliferation and signaling ability of immortalized human meibomian gland (iHMGECs), corneal (iHCECs) and conjunctival (iHConjECs) epithelial cells. We hypothesize that these preservatives, at concentrations (BAK = 1 mg/ml FA = 0.74 mg/ml) approved for consumer use, are toxic to human ocular surface and adnexal cells. These preservatives include benzalkonium chloride (BAK) and formaldehyde (FA)-releasing preservatives. Our results support our hypothesis and demonstrate that the cosmetic preservatives, BAK and FA, exert many toxic effects on cells of the ocular surface and adnexa.Ĭosmetic products, such as mascara, eye shadow, eyeliner and eye makeup remover are used extensively to highlight the eyes or clean the eyelids, and typically contain preservatives to prevent microbial growth. Cellular signaling, as indicated by AKT phosphorylation after 15 (FA) or 30 ( BAK) minutes of treatment, was also reduced in a dose-dependent fashion in all 3 cell types, irrespective of whether cells had been cultured under proliferating or differentiating conditions. Many of the concentrations tested induced cell atrophy, poor adherence, decreased proliferation and death, after 5 days of exposure. Our results demonstrate that BAK and FA cause dose-dependent changes in the morphology, survival, proliferation and AKT signaling of iHMGECs, iHCECs and iHConjECs. Experimental procedures included analyses of cell appearance, cell number, and neutral lipid content (LipidTox), lysosome accumulation (LysoTracker) and AKT signaling in all 3 cell types. We used low BAK levels, because we found that 0.5 mg/ml and 50 μg/ml BAK killed iHMGECs within 1 day after a 15 min exposure. iHMGECs, iHCECs and iHConjECs were cultured with different concentrations of BAK (5 μg/ml to 0.005 μg/ml) or FA (1 mg/ml to 1 μg/ml) under basal, proliferating or differentiating conditions up to 7 days. Accordingly, we tested the influence of BAK and FA on the morphology, survival, and proliferation and signaling ability of immortalized human meibomian gland (iHMGECs), corneal (iHCECs) and conjunctival (iHConjECs) epithelial cells. We hypothesize that these preservatives, at concentrations ( BAK = 1 mg/ml FA = 0.74 mg/ml) approved for consumer use, are toxic to human ocular surface and adnexal cells. These preservatives include benzalkonium chloride ( BAK) and formaldehyde (FA)-releasing preservatives. Below, discover Erika Jayne’s favorite beauty products, some of which you might consider travel staples yourself.Cosmetic products, such as mascara, eye shadow, eyeliner and eye makeup remover are used extensively to highlight the eyes or clean the eyelids, and typically contain preservatives to prevent microbial growth. With Erika well on her way to becoming a hair mogul, we wanted to discover what she always has handy to keep her hair and complexion looking so damn perfect everywhere she goes. Given we love all things hair and Erika, it’s a no-brainer that we’re already fans of her luxury hair extensions line even though it just launched. And that is absolutely the case with her latest venture, Pretty Mess Hair. Whether she’s performing her newest hit single or gallivanting with The Real Housewives of Beverly Hills gals, there’s something so captivating about Erika that draws us in with everything she does. We were curious what those coveted products were for some of our favorite people-so… we asked. Forgetting to stash them in our carry-on could easily ruin any trip. There is a long list of hair products we love, but not all of them are ones we wouldn’t dare forget to pack for a trip. ![]()
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